Figure 1

Endogenous expression of GRP78 in hepatocellular carcinoma cells. (A) Quantative RT-PCR analysis for mRNA levels of GRP78, MMP-2, MMP-9, MMP-14, and TIMP-2 in hepatocellular carcinoma cell lines SMMC7721 and HepG2. The mRNA contents in the cells were presented as the relative levels normalized to 18 S mRNA. (B) Western blot analysis for protein levels of GRP78, MMP-2, MMP-9, MMP-14, and TIMP-2 in hepatocellular carcinoma cell lines SMMC7721 and HepG2. Protein levels were expressed as the ratio of target protein over β-actin. All the experiments were repeated for three times, the values were presented as $\overline{x}$± SE and analyzed by the students’ t-test. (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels).