Figure 6
JNK signaling is involved in the reduced MMP-2 activity caused by GRP78 Knockdown. (A). Gelatin zymograph analysis of MMP-2 activity in SP600125 treated GRP78 overexpressing cells (OE). GRP78 OE cells were treated with the JNK inhibitor SP600125 at various concentrations for 12 h, the conditioned medium were collected and the activity of MMP-2 were detected by gelatin zymograph. Schematic diagram of the MMP-2 activities in GRP78 OE cells that treated with SP600125, the results of quantative analysis were represented as ± SE and analyzed by one-way ANOVA. (Columns, mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels). (B) Western blot analysis of the c-Jun and p-c-Jun in the cells that stably expressing shGRP78-3 and schematic diagram of the expression levels of c-Jun and p-c-Jun. All the experiments were repeated for three times, the results of quantative analysis were represented as ± SE and analyzed by one-way ANOVA. (Columns, mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels).