Table 1 Sequences of the primers used for qPCR of transcripts coding for SGK1 (all four isoforms), for each of the four isoforms and for glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Gene Symbol | Accession Number | Sense Primer | Antisense Primer |
|---|---|---|---|
SGK1 (all 4 isoforms) | N/A | AGGGCAGTTTTGGAAAGGTT | CTGTAAAACTTTGACTGCATAGAACA |
SGK1 (isoform 1) | NM_005627.3 | GGCACCCTCACTTACTCCAG | GGCAATCTTCTGAATAAAGTCGTT |
SGK1 (isoform 2) | NM_001143676.1 | CGGTGGAAAATGGTAAACAAA | CTTGATCCACCTTCGTACCC |
SGK1 (isoform 3) | NM_001143677.1 | GAAGCTATAAAACCCCCTTTGAA | GGCAATCTTCTGAATAAAGTCGTT |
SGK1 (isoform 4) | NM_001143678.1 | CTTCCTGCTGAGCGGACT | GGCAATCTTCTGAATAAAGTCGTT |
GAPDH | NM_002046 | AGCCACATCGCTCAGACA | GCCCAATACGACCAAATCC |