Figure 3
From: Claudin-5 is involved in breast cancer cell motility through the N-WASP and ROCK signalling pathways
Confirmation of over-expression and knockdown in MDA-MB-231 cells. (a) Screening of different human tissues for Claudin-5 coding sequence at mRNA level using RT-PCR. β-actin is used as a loading control. The placenta tissue was selected as a template. (b) Verification of Claudin-5 over-expression and knockdown in MDA-MB-231cells. Claudin-5 levels were higher in MDA-MB-231 CL5exp compared to the controls, as seen at mRNA level using RT-PCR. Claudin-5 expression was reduced in MDA-MB-231 CL5rib2 when ribozyme 2 was used, at mRNA level using RT-PCR. (c) Protein level using Western blot analysis to show expression of Claudin-5. (d) Immunofluorescence staining showing the distribution of Claudin-5 in Overexpressing cells (left) with Phalloidin to show actin (centre) and merged (right).