Figure 2

MiR-20a restoration in HCC cell lines inhibit proliferation and block cell cycle progression in vitro (A) and (B) Validation of miR-20a level in SMMC-7721 and HepG2 cells upon transfection with miR-20a precursor. (C) Proliferation assay of HCC cell lines in response to miR-20 restoration. HepG2 or SMMC-7721 cells were seeded into 96-well plates and incubated in the presence of miR-20a precursor or control oligonucleotide. Cell proliferation assay was done after culturing for 72 h. The experiment was done in triplicate. (D) and (E) Influence of miR-20a on cell cycle progression of HCC cell lines. SMMC-7721 and HepG2 cells were transfected with miR-20a precursor. Cell cycle analysis was performed by flow cytometry. Data are given as mean ± SD of three independent experiments. Asterisks indicate statistical significance of differences in the number of cells in G1, S, or G2/M between miR-20a precursor transfected and control oligo transfected cells (P < 0.05; Student’s t test).