Table 1 Questionnaire results from the 16 participant centers
| Â | N (%) |
|---|---|
N° annual HER2 determination |  |
<100 | 4 (25%) |
100-500 | 10 (62.5%) |
>500 | 2 (12.5%) |
Material | Â |
Paraffin embedded tissue | 16 (100%) |
Type of fixation | Â |
Buffered formalin | 12 (75%) |
Formalin | 2 (12.5%) |
Other | 2 (12.5%) |
Time of tissue fixation | Â |
12Â hours | 2 (12.5%) |
24Â hours | 10 (62.5%) |
Other | 4 (25%) |
Time from cutting to IHC | Â |
24Â hours | 10 (62.5%) |
1Â week | 4 (25%) |
other | 2 (12.5%) |
Slide storage | Â |
37°C | 5 (31%) |
Room temperature | 11 (69%) |
Immunostaining procedure | Â |
Automated | 11 (69%) |
Manual | 5 (31%) |
Type of reagent | Â |
A0485 PoAb | 11 (69%) |
CB11 MoAb | 4 (25%) |
Herceptest | 1 (6%) |
Chromogen | Â |
DAB | 16 (100%) |
Evaluation | Â |
Optical microscope | 15 (94%) |
Optical microscope + Image analyzer | 1 (6%) |
Evaluation criteria | Â |
Score and % of positive cells | 10 (62.5%) |
Score | 6 (27.5%) |