Figure 2
From: EZH2 knockdown suppresses the growth and invasion of human inflammatory breast cancer cells
High tumorigenicity correlates with high expression of EZH2 in FC-IBC-02 cells. A. Initial FC-IBC-02 cells obtained from pleural effusion fluid of IBC patient growing as suspension (FC-IBC-02S) and adherent (FC-IBC-02A) populations (×200 magnification). Tumor spheroids exhibit budding from parent to daughter spheroid in suspension culture. B. Tumor spheroid growth of FC-IBC-02S and FC-IBC-02A cells in suspensions under ultra-low attachment culture condition for 6 days (×100 magnification). C. Expression of EZH2 in suspension and adherent populations of FC-IBC-02 cells was examined by immunoblot. D. Growth of FC-IBC-02 adherent (2 × 106) and suspension (2 × 106) cells in vivo in SCID mice. Tumor volume was monitored over time, and the tumor was excised and weighed after 7 weeks. *, P < 0.001 compared to FC-IBC-02A at week 7.