Figure 1

Melanosphere isolation and validation. A) Image of melanospheres (left) and their differentiated progeny (right). B) Tumor volumes of xenografts generated by spheres or differentiated (diff) melanoma cells injected subcutaneously in Nude mice at the indicated cell doses. Mean ± SD of 3 independent experiments is shown. ** p < 0,01. C) Table of melanospheres tumorigenicity in dose response experiments. Cell numbers, number of mice injected and percentage of tumor engraftment is indicated for each condition. Tumors were monitored for 8 weeks post-injection. D) Hematoxylin and eosin (H&E) or immunohistochemistry for the indicated antigens performed on patient tumor or xenograft generated by melanospheres. The original magnification of each image is indicated.