Figure 1

Arsenic trioxide induces oxidative stress in HL-60 cells. (A) HL-60 cells were incubated with 2, 4, 6 and 8 mg/ml of ATO for 24 hrs and the level of malondialdehyde(MDA) was measured by spectrophotometry at 532 nm. MDA was expressed in nmole/ml. Data represent the means of three independent experiments ± SDs (# P < 0.05). (B) Cells were treated with different doses of ATO for 24 hrs and reduced GSH level was measured by spectrophotometry at 412 nm. GSH was expressed in nmole GSH/ml. Data represent the means of three independent experiments ± SDs (##P < 0.05). (C) HL-60 cells were grown in absence or presence of different doses of ATO for 24 hrs and DNA damage was analyzed by alkaline Comet assay. (D) ATO – induced genotoxicity was expressed as percentage of DNA damage. Data represent the means of three independent experiments ± SDs (**P < 0.01). (E) ATO-induced comet tail length was measured in micrometer. Data represent the means of three independent experiments ± SDs (***P < 0.01).