Figure 1From: Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemiaRestriction analysis of DNMT3A R882H mutations. 1) Agarose gel analysis of restricted products of 5 positive (12, 34, 57, 65, 187) and 2 negative (54, 143) patients. Wt samples showed 2 bands at 190Â bp and 114Â bp. Positive samples showed 3 bands at 289Â bp, 190Â bp, 114Â bp because of the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as the marker. 2) Representative sequence analysis of patient 187 showing heterozygote mutation CGC to CAC.Back to article page