Figure 3From: Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemiaHRM analysis of DNMT3A mutations. 1) Difference plot for HRM analysis of DNMT3A R882H G>A and R882X G>C mutations. Normalisation was performed to the wt allele. R882X showed a right-shifted peak compared to R882H. 2) Melting curve profiles of DNMT3A R882H G>A, R882X G>C and wt allele. Vertical axis corresponds to changes in the fluorescence signal over time (dF/dT). R882H G>A displayed 2 peaks (84.5°C and 85.6°C), while the wt allele had only one peak at 85.7°C. R882X G>C had a left shifted peak at 85.6°C.Back to article page