Figure 8

Sensitivity analysis of different IDH1 mutations. 1) Difference plot for HRM analysis of serial dilutions of IDH1 G105 C>T: Undiluted mutation ratio was 51.9% (estimated by sequencing). Correct estimation was possible up to a mutation ratio of 7.8%; lower mutation ratios were identified false-negative. Normalisation was performed to the R132S C>A allele. 2) Difference plot for HRM analysis of serial dilutions of IDH1 R132C C>T: Undiluted mutation ratio was 44.6% (estimated by sequencing). Correct estimation was possible up to a mutation ratio of 6.69%; lower mutation ratios were identified false-negative. Normalisation was performed to the R132S C>A allele. 3) Difference plot for HRM analysis of serial dilutions of IDH1 R132S C>A: Undiluted mutation ratio was 40.4% (estimated by sequencing). Correct estimation was possible up to a mutation ratio of 6%, lower mutation ratios were identified false-negative. Normalisation was performed to the G105 C>T allele.