Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemia

Table 1 Comparative characteristics of all the methods used in this study

	DNMT3A			IDH2			IDH1
	Restriction endonuclease	HRM	Sanger sequencing	ARMS	HRM	Sanger sequencing	HRM	Sanger sequencing
Sensitivity*, %	0.05	5.9	10	4.5	4.5	10	6 to 7.8	10
Turnaround time, days	1	1	2 to 3	1	1	2 to 3	1	2 to 3
Technician time, hours	4	3.5	10 to 12	3	3.5	10 to 12	3.5	10 to 12
Cost of diagnosis method, €	32.13	28	122	44.16	28	122	28	122
Interpretation	Easy	Medium -difficult	Medium	Easy	Medium -difficult	Medium	Medium -difficult	Medium
Identification of different/rare mutations	No	Yes	Yes	No	Yes	Yes	Yes	Yes
Special equipment	PCR cycler	HRM real time PCR cycler	Sequencer	PCR cycler	HRM real time real time PCR cycler	Sequencer	HRM real time real time PCR cycler	Sequencer

*Sensitivity was measured as the minimal percentage of mutated allele in a sample detected by the assay.

ISSN: 1756-9966