Figure 6From: Early B-cell factor 3 (EBF3) is a novel tumor suppressor gene with promoter hypermethylation in pediatric acute myeloid leukemia Overexpression of EBF3 inhibited proliferation and induced apoptosis in leukemia cells. (A) Western blot analysis of EBF3 expression in EBF3 transfected leukemia cells. Transfection with EBF3 lentivirus PLVX-EBF3 significantly upregulated expression of EBF3 in AML cells compared with mock-transfected cells. An expression level of cleaved PARP, a marker of apoptosis, was analyzed by Western blotting. (B) CCK-8 assays show that transfection with EBF3 lentivirus inhibits proliferation in HL-60 and MV4-11 cells compared with mock-transfected cells. (C) The number of cells displaying apoptotic features is higher in the HL-60 and MV4-11 cells transfected with PLVX-EBF3 compared with the mock-transfected cells. (D) Quantification shows that the proportion of apoptotic cells in the EBF3-overexpressing cells (PLVX-EBF3) was significantly greater than the vector control group (PLVX-Ve). **P < 0.01.Back to article page