Fig. 3

ECM-related gene expressions of HOSS1 cells in 3D and 2D cultures. a: RT-qPCR analysis of the expression of ECM-related genes in 3D and 2D cultures of HOSS1 cells at day 8. Data are presented as fold differences relative to 2D-cultured cells for each gene, which was defined as 1 (calibrator). Data are mean ± SD of 3 independent experiments with Student’s t-test. Error bars indicate SD. b: Western blotting of ECM-related protein expression in 3D and 2D cultures at day 8