Fig 1

Gross and microscopic appearance of colon mucosa in (1) control group, (2) AOM group, (3) 5-FU group, (4) vitamin D group and (5) 5-FU/D group. Colonic mucosa was examined by (Panel a): Gross; (Panel b): dissecting microscopy following staining with 0.2 % methylene blue and (Panel c): light microscopy at magnifications ×100 and ×200 following staining with H&E. (Black arrow head = tumour observed by naked eye; red star = large ACF [>4 crypts/focus]; light blue arrow = hyperplasia and green arrow = dysplasia)