Fig. 3

Effects of Loc554202 on the cell cycle and apoptosis of colorectal cancer cells in vitro. HCT116 and DLD1 cells were transfected with pCDNA-Loc554202 or empty vector. a and b The bar chart represents the percentage of cells in the G0/G1, S, or G2/M phases, as indicated. c and d The percentage of apoptotic cells was determined by a flowcytometric analysis. The data represent the means ± S.D. from three independent experiments. e The level of apoptosis in HCT116 and DLD1 cells after they were transfected with pCDNA-Loc554202 or empty vector as determined by TUNEL staining.*P < 0.05