Fig. 3From: Crizotinib-induced antitumour activity in human alveolar rhabdomyosarcoma cells is not solely dependent on ALK and MET inhibitionChanges in molecular regulators of cell cycle in RH4 and RH30 cell lines treated with crizotinib. a Immunofluorescence experiments showing the expression and localization of p21, Cdc25C, Cdc2, Cyclin B1 and p-Cdc2 (Thr14/Tyr15) proteins in RH4 cells treated or not with 1.5 μM crizotinib for 24 h. b Immunofluorescence experiments showing the expression and localization of p21, Cdc25C, Cdc2, Cyclin B1 and p-Cdc2 (Thr14/Tyr15) proteins in RH30 cells treated with 1.5 μM crizotinib for 24 h. Control cells were treated with DMSO. TRITC-phalloidin was used to mark F-actin filaments of cellular cytoskeleton. Images captured under ApoTome microscope at 40× magnificationBack to article page