Fig. 3

ROR1 is a direct downstream target gene of miR-27b-3p. a The ROR1 3’UTR regions containing the wild-type or mutant binding site for miR-27b-3p are shown. b Western blotting was used to analyze the expression levels of ROR1 after transfecting with miR-NC or miR-27b-3p mimics. c Relative ROR1 luciferase activity was analyzed after the wild-type or mutant 3’UTR reporter plasmids co-transfected with miR-NC or miR-27b-3p. d RT- PCR was used to detect the expression levels of miR-27b-3p in GC and non-GC specimens. e The scatter plots show the expression levels of miR-27b-3p and ROR1 in GC samples. Linear regression analysis was used to measure the association between miR-27b-3p and ROR1. f The expression of miR-27b-3p in a panel of tumorigenic GC cell lines