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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: MiR-302a/b/c/d cooperatively sensitizes breast cancer cells to adriamycin via suppressing P-glycoprotein(P-gp) by targeting MAP/ERK kinase kinase 1 (MEKK1)

Fig. 1

Downregulation of miR-302 expressions in MCF-7/ADR cells. a Cells were treated with various concentrations of ADR, PAC or VP-16. Survived cells were measured by MTS assay. MTS assay shows that MCF-7/ADR cells are much more resistant to ADR, PAC and VP-16 than MCF-7 cells (b) Left: Western blotting analysis showing the protein expression of MRP, P-gp, LRP, and BCRP in MCF-7 and MCF-7/ADR cells. GAPDH was used as an internal loading control. Right: Densitometric analysis for the detected protein expression. c Sequence alignment of human miR-302 family miRNAs. d qRT-PCR indicates a significant down-regulation of miR-302 in MCF-7/ADR cells compared with MCF-7 cells. All graphs show means ± S.D. of three independent experiments

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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