Fig. 7

miR-133b participates in cell cycle regulation by targeting PPP2R2D. a-c After transfection with miNC or 133mi, HepG2 cells were treated with or without 2.5 μg/ml cDDP for 12 h. a miR-133b and PPP2R2D mRNA levels. b Protein levels of B55δ, Cyclin B1, and Cyclin E1. c Cell cycle distribution was analyzed by FCM. d-f HepG2 cells transfected with inNC or 133in were treated with or without cDDP. d qRT-PCR, e WB, and (f) FCM were conducted as before. The data are expressed as mean ± SD of three independent experiments. *P < 0.01 as compared with Ctrl group of miNC- or inNC-transfected HepG2 cells; ^# P < 0.01 as compared with cDDP group of miNC- or inNC-transfected HepG2 cells