Fig. 4

Cancer patient sera permanently transformed BRCA1-KO fibroblasts. a BRCA1-KO fibroblasts were treated with cancer patient serum for 2 weeks. Cells were then transferred to regular culture medium (10 % FBS-supplemented DMEM-F12 medium with 1 % antibiotics) for 2 weeks. Cells were injected subcutaneously into NOD/SCID mice. 4 weeks after cells transplantation, mice were euthanized and the tumors photographed. Representative pictures are shown. b Formalin-fixed paraffin-embedded xenotransplants samples were processed for H&E staining