Experimental model | Drug | Route of administration | Dose | Treatment regimen |
---|---|---|---|---|
Experimental metastasis (Fig. 1) | 1-MNA | Per os in drinking water | 100 mg/kg/day | Treatment initiated 7 days prior cancer transplantation and continued to the end of the experiment |
1,4-DMP | ||||
Spontaneous metastasis (single drug treatment) (Fig. 2) | 1-MNA | Per os in drinking water | 100 mg/kg/day | Continuously from the 7th day of the experiment to the end of the experiment |
1,4-DMP | ||||
Matrigel plugs (Fig. 3) | 1-MNA | Per os in drinking water | 100 mg/kg/day | Continuously starting from the day of matrigel plugs transplantation |
1,4-DMP | ||||
Cyclophosphamide | intraperitoneally | 25Â mg/kg/day | On days 0, 3, 5 | |
1-MNA | Per os in drinking water | 100Â mg/kg/day | Continuously from the 1st day of the experiment to the end of the experiment | |
1,4-DMP | ||||
Cyclophosphamide | Intraperitoneally | 25Â mg/kg/day | From the 7th day of the experiment, 3 times each week |