Fig. 4

ATO Up-Regulates the Expression of miR-125b by Demethylation of DNA in Chondrosarcoma Cells. a HCS-2/8, OUMS-27 and SW1353 cells were exposed to 1.5 μM ATO for 24 h. qRT-PCR analyses of miR-125b levels (mean ± SD, n = 3). **P < 0.01 compared with control cells. b A pathway showing the demethylation effects induced by As³⁺. As³⁺MT (As³⁺ methyltransferase), MMA, monomethylarsinic acid; DMA, dimethylarsinic acid. c BSP shows that the miR-125b methylation ratio of the 7 CpG loci became 57.1 % (4/7) and 42.8 % (4/7) from 100.0 % in HCS-2/8 and OUMS-27 cells, respectively