Fig. 1From: Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathwaysILK and RI expression is determined by Western blot and Immunofluorescence after transfection for 48 h. a Immunofluorescent observation of ILK and RI was detected respectively. EJ-ILK cells demonstrated remarkably stronger immunofluorescent signal in cytoplasm whereas EJ-RI cells demonstrated significantly stronger immunofluorescent signal in nucleus, compared with the other control groups respectively (magnification × 200). b & c ILK and RI protein expression level was evaluated by Western blot. Proteins were separated by SDS-PAGE and then binded to specified monoclonal ILK antibodies or polyclonal RI antibodies. β-actin was used as control. The levels of ILK proteins were remarkably increased by transfected with pCMV-3 × FLAG-ILK and the levels of RI proteins were significantly increased by transfected with lentivirus containing RNH1 (LV-RNH1), compared with the control groups respectivelyBack to article page