Fig. 2From: Spheres derived from the human SN12C renal cell carcinoma cell line are enriched in tumor initiating cellsTumorigenecity of spheroid and adherent cells in vivo. a1 Representative xenograft tumors derived from 106,107 adherent cells and 104,105,106 spheroid cells. a2 BALB/C mice injected with different number of spheroid cells and adherent cells that developed tumors and the tumor volumes were quantified. b Representative xenograft tumors derived from 105 cells. All mice formed tumors with spheroid cells, but not with adherent cells. c The H&E staining, immunostained with rat anti-mouse and mouse anti-human CD34 antibody of the tumors formed by spheroid cells and adherent cells (magnification × 100). Representative the blood microvessel density(MVD) and vasculogenic mimicry (VM) in tumors derived from spheroid cells and adherent cells was measured. The experiment has been repeated once with a similar outcome. (Magnification × 100). Representative staining of three independent experiments was shownBack to article page