Fig. 5
Representative chart of flow cytometric analysis of monocyte-derived DCs. DCs were harvested on day 7 and analyzed by flow cytometry using monoclonal anti-CD14, HLA-DR, CD80, CD86 and CD83 antibodies and respective isotype controls. Flow cytometric analysis of DCs treated with NP-containing antigen showed that expression of CD80, CD86, HLADR, CD83 was up-regulated, while expression of CD14 surface marker was down-regulated in comparison to DCs pulsed with tumor lysate alone (P ≤ 0.05) Depicted data are averaged from three independent samples obtained from breast cancer patient (n = 3) (*: indicates the statistical significance)