Fig. 6

Combining GANT61 and TMZ treatment reversed EMT of glioma cells. a The mRNA expression levels of different genes. qRT-PCR analysis demonstrated that either GANT61 or TMZ treatment resulted in a reduction of N-cadherin, Vimentin and Fibronectin mRNA expression and an elevation of E-cadherin mRNA expression. The combination of GANT61 and TMZ treatment led to more significant decreases in N-cadherin, Vimentin and Fibronectin mRNA expression and increase in E-cadherin mRNA expression than cells treated with GANT61 or TMZ treatment alone. *P < 0.05, **P < 0.01, ***P < 0.001. b Western blot analysis showed that the protein expression of N-cadherin, Vimentin and Fibronectin decreased, and the protein expression of E-cadherin increased in cells, especially in the GANT61 + TMZ group, while only a slight change was detected in the TMZ group. This experiment was repeated three times