Fig. 1

TY-011 inhibits Aurora A and Aurora B kinases activities. a Chemical structure of TY-011. b The HTRF® KinEASE™-STK kit was used to measure inhibition of Aurora A and Aurora B activities by TY-011. The inhibition rates were determined in reference to the control. Data presented are the mean ± SD of three independent experiments. c Effects of TY-011 on phosphorylation of Aurora A and Histone H3 were analyzed by western blot. MGC-803 cells synchronized with nocodazole (100 nM) for 16 h were treated with various concentration of TY-011 for 24 h, then were lysed and subjected for western blot. The results are representative of three independent experiments. d Effects of TY-011 on phosphorylation of Aurora A and Histone H3 were analyzed by immunofluorescence. MGC-803 cells were treated with TY-011 (0.8 μM) for 24 h, then fixed and stained for p-Aurora A (T288) (green) or p-Histone H3 (H10) (green), MPM2 (red) and DNA (blue). MPM2 was used to locate the mitotic cells. The images are representative of two independent experiments