Fig. 5

Fbw7 inhibits downstream Stat3 target genes that block apoptosis. a-d, qPCR analysis of the relative mRNA expression in ABC-DLBCL cell lines at the indicated time intervals. mRNA levels were normalized to the geometric mean of the housekeeping gene β-actin and calculated using the 2^−ΔΔCT method. The experiments were repeated three times. Statistical analysis was performed using a two-tailed unpaired Student t test.*, P < 0.05; **, P < 0.01; ***, P < 0.001. a (right), cells were transfected with Fbw7 and compared with vector control to detect Fbw7 expression. b (left), cells were transfected with Fbw7 and compared with vector control to detect Stat3 expression. c and d, qPCR analysis of the relative Myc, Survivin, Mcl-1, Pim-1, Bcl-2 and Bcl-xl mRNA levels after transfecting Fbw7 for 48 h in SU-DHL-2 and OCI-LY-3 cells, respectively. e siRNA of Stat3, plasmids of Fbw7 and Stat3 was transfected as instructed for 24 h in ABC-DLBCL cell lines. And then cell lines were cultured with Doxorubicin (1.0 μM) for 24 h to induce apoptosis before flow cytometry. Statistical significance was determined by a two-tailed, unpaired Student t test. ***, P < 0.001