Fig. 4
Hypoxia inhibits miR-519d expression in NPC cells. a, b NPC HNE-1 and HONE-1 were incubated for 24 h with media containing 0 or 100 μM CoCl2, respectively, and expressions of HIF1α protein (a), as well as miR-519d (b), were examined. c to e) HNE-1 and HONE-1 cells expressing empty vector and overexpressing HIF1α, respectively, were subjected to analyses of HIF1α mRNA (c) and protein (d), as well as miR-519d (e) levels. Values were expressed as mean ± SD. * p < 0.05, ** p < 0.01, compared to control. # p < 0.05, ## p < 0.01, compared to empty vector