Fig. 5

CIP4 plays an important role in invadopodia formation in NPC cells. a and b 5-8F cells and S18 cells transfected with control or CIP4 siRNA were plated on glass-bottom dishes coated with Gelatin From Pig Skin, Oregon Green® 488 Conjugate and cultured for 20 h before being stained for endogenous CIP4 and F-actin. The cells were fixed and stained with anti-cortactin antibody (blue) and F-actin (red). The arrowheads indicate the position of extracellular matrix (ECM)-degrading invadopodia. Scale bar, 30 μm. c and d Gelatin degradation areas were counted and measured, normalized for cell number and averaged over replicates from three independent experiments. The data are expressed as the mean ± S.D. of three independent experiments. ***, P < 0.001