Fig. 5

Effect of RES on DOX-induced EMT. SGC7901/DOX cells were untreated or treated with 1 mg/l DOX, 50 mg/l RES or 1 mg/l DOX simultaneously combined with 50 mg/l RES for 48 h. a Representative images of treated cells were shown. Scale bar represents 10 μm. b, c After 48 h treatment, cells were harvested for Western blot analysis to detect whether DOX and RES affect the expression of EMT-related proteins. Bar diagram shows the relative expressions of proteins normalized to GAPDH. Data are represented as mean ± SD of three independent (n = 3, *** p < 0.001; ** p < 0.01; * p < 0.05: NS means not significant, p > 0.05). d Immunofluorescence assay was used to detect EMT-related proteins. Vimentin, E-cadherin and β-catenin were stained red and nuclei stained with DAPI were blue. The white arrow indicates β-catenin nuclear tranlocation. Images were captured at 1800× magnification