Fig. 5
From: Regulation of TRIM24 by miR-511 modulates cell proliferation in gastric cancer
Overexpression of TRIM24 partially rescues miR-511-induced effects on GC cell proliferation. a Western blot analysis of TRIM24 protein expression after transfection of MGC803 and HGC-27 cells with miR-511 mimics or miR-511 mimics and TRIM24-no UTR (TRIM24 without its 3′-UTR, shown as TRIM24). b At the indicated time points post-transfection, the cell growth rate was evaluated using the CCK8 assay. c Colony formation assay analysis of GC cells after indicated treatments. d Representative micrographs (left) and quantification (right) of EdU incorporating-cells after transfection with miR-511 mimics, or miR-511 mimics and TRIM24-no UTR. e The cell cycle distribution was detected by flow cytometric analysis at 48 h post-transfection. (*p < 0.05, **p < 0.01; n = 3)