Fig. 5

High iASPP and low FBXL5/BTG3 expression correlates with poor survival in CC patients. a The mRNA expression of FBXL5, BTG3, Vimentin and E-cadherin was assessed by qPCR analysis in 40 paired cancerous and normal tissue samples of CC patients. b Pan-cancer analysis of iASPP, FBXL5 and BTG3 mRNA expression in human cervical cancer tissues and adjacent normal tissues was performed using the BioExpress database. c Correlative analysis of the indicated mRNAs and miR-20a in 40 paired tumor and non-tumor tissues of CC patients. The expression of mRNAs and miR-20a was determined using qPCR. Relative expression of each mRNA and miR-20a in tumor tissues was calculated as a fold change compared to that of corresponding non-tumor tissues. Spearman correlation coefficient with the respective significance is shown. d Kaplan-Meier survival curve of 191 TCGA (Cancer Genome Atlas database) cervical cancer samples was created using the SurvMicro database, based on the low or high risk for a poor outcome (left). Box plots demonstrating significantly higher level of iASPP and lower level of FBXL5/BTG3 in the high-risk CC patients (right). e Schematic representation of the proposed mechanism by which iASPP promotes EMT and cisplatin resistance in CC via miR-20a-FBXL5/BTG3 signaling. **P < 0.01