Fig. 3

M2-exos induce the resistance of MFC cells to DDP in vitro and in vivo. a Cell viability was assessed by CCK-8 assays. M2-derived conditioned medium (CM) or exosomes (M2-Exo) attenuated DDP-induced cell suppression. MFC cells were pretreated with M2-derived CM or exosomes, and then exposed to DDP for 48 h. b Flow cytometric analyses of apoptotic cells. MFC cells were exposed to DDP alone (ctrl) or DDP and M2-Exo for 48 h. The quantitative data are presented as the mean ± SD of triplicate experiments. (*** p < 0.001). c Tumor growth curves in nude mice inoculated with MFC cells that were pretreated with or without M2-Exo.DDP or PBS (ctrl) treatment was initiated 10 days after subcutaneous injections of tumor cells. (n = 5, * p < 0.05). d The size of tumors at the end of the experiment from mice treated with PBS (ctrl), DDP, M2-Exo + DDP. (n = 5). e The mean weight of tumors from mice shown in (d). (n = 5, ** p < 0.01)