Fig. 5

β-catenin is involved in FN-induced bladder cancer cell proliferation. a β-catenin, c-myc, and CyclinD1 expression levels in T24 and J82 cells were examined by western blotting after FN treatment (0, 5, 10 and 20 μg/mL) for 48 h. b T24 and J82 cells transfected with β-catenin siRNA or control siRNA were treated with FN (0 and 20 μg/mL) for 2 weeks and allowed to form colonies. c and d The viability of β-catenin siRNA or control siRNA transfected T24 and J82 cells which were incubated with FN (0 and 20 μg/mL) for 4 days was evaluated by using MTT assay (**P < 0.01, ***P < 0.001). e and f Cell cycle distribution of β-catenin siRNA or control siRNA transfected T24 and J82 cells treated with FN (0 and 20 μg/mL) for 48 h was evaluated by flow cytometry. The percentage of cells in each phase were shown (*P < 0.05, **P < 0.01, ***P < 0.001). g After 72 h transfection with β-catenin siRNA or control siRNA, T24 and J82 cells were treated with FN (0 and 20 μg/mL) for 48 h, and western blotting analysis of β-catenin, c-myc, and CyclinD1 expression levels were performed