Fig. 1

Consequence of IDH1 mutation in HT-1080. The detected 2-HG peak on chromatogram (LC-MS) (a.) and the heterozygous IDH1 gene mutation which resulted IDH1 R132C was confirmed by sequencing (Sanger) (b.); The main sources of 2-HG production were evaluated by ¹³C incorporations in 2-HG after one-hour U-¹³C-glucose or U-¹³C-glutamine labelling and LC-MS (representative data) (c.). t_r: retention time; m + 0 = unlabelled metabolites; m + 1/2/3 = 1/2/3 ¹³C in 2-HG from glucose, m + 5 = 5 ¹³C in 2-HG from glutamine, (m + 4 and m + 5 isotopomers were not detectable in these cells after one-hour glucose labelling in 2-HG)