Fig. 5From: In vitro and in vivo cytotoxicity of troglitazone in pancreatic cancerEffects of TGZ on cell invasion and cell migration. a The cell invasion assay was performed using 24-well BD BioCoatā¢ MatrigelĀ® invasion chambers with 8.0-Ī¼m polycarbonate membrane filters. Cells were seeded on membranes at 2āĆā105 cells/well with FBS-free medium, after which the membranes were placed into the lower chamber and incubated with 10% FBS-containing medium. After culture with or without TGZ (MIA Paca2: 10Ā Ī¼M, PANC-1: 1Ā Ī¼M) for 24Ā h, cells on the upper surface of the membranes were removed using a cotton swab. Invasive cells that penetrated through the pores and migrated to the underside of the membrane were stained with Giemsa solution after fixation with 100% methanol. Cell number was quantified under microscopy. b The cell migration assay was conducted in the same manner as the cell invasion assay, except for the use of non-coated chambers. Data represent the meanā+āS.D. from three or four independent preparations. * pā<ā0.05 vs. control (t-test). TGZ, troglitazone; FBS, fetal bovine serumBack to article page