Fig. 4
From: Antibody targeting of claudin-1 as a potential colorectal cancer therapy
In vitro effects of the 6F6 mAb on CRC cell survival and growth. a Clonogenic assay in CLDN1-positive CRC cells in the presence or not (NT) of 100 μg/ml of the 6 F6 mAb. Images were obtained using a Celigo™ imaging cytometer. Scale bar: 5 mm. b Quantification of the clonogenic assay results: the histogram shows the percentage of colonies in treated cultures (i.e., the ratio between the number of colonies in the treated well and the number of colonies in the untreated well × 100); * = p < 0.05 (paired t-test). c Effect of the 6F6 mAb on growth of 3D spheroids. Cells were incubated or not (NT) with 100 μg/ml of 6F6 or irrelevant (IRR) mAb. Representative images of spheroids after 72 h of culture on Ultra-low attachment plates. d Bioluminescence cytotoxicity assay to determine cell viability in spheroids grown in the presence or not (NT) of the 6F6 or an irrelevant (IRR) mAb. Cell viability was assessed by measuring the ATP content. Results are shown as the ratio between the ATP content in treated spheroids and the ATP content in untreated spheroids × 100; * = p < 0.05; ** = p < 0.01; *** = p < 0.001 (paired t-test)