Fig. 5

Ectopic expression of miR-30c or gain and loss of CTHRC1 affects breast cancer cell proliferation, apoptosis, invasion and migration. a The effect of ectopic expression of miR-30c or gain and loss of CTHRC1 on cell viability was detected by CCK-8 assay. *P < 0.05, **P < 0.01. b The effect of ectopic expression of miR-30c or gain and loss of CTHRC1 on cell proliferation was measured by colony formation assay. Representative images of colony formation are shown (left panel). Results represented the mean ± SD in triplicate using bar graph (right panel). **P < 0.01. c The effect of ectopic expression of miR-30c or gain and loss of CTHRC1 on cell cycle progression was measured by flow cytometry analysis. *P < 0.05, **P < 0.01. d The effect of ectopic expression of miR-30c or gain and loss of CTHRC1 on cell apoptosis was detected by flow cytometry analysis. e The effect of ectopic expression of miR-30c or gain and loss of CTHRC1 on cell invasion and migration was measured by transwell invasion/migration assay. Representative images are shown (left panel). Magnification: ×100. Results represented the mean ± SD in triplicate using bar graph (right panel). **P < 0.01