Fig. 8

Depletion of miR-193a-5p reduces PC xenograft growth in vivo. a PC3 cells engineered to stably express anti-miR-193a-5p (LV-anti-miR-193a-5p) or negative control (LV-miR-Ctl) were injected subcutaneously in 200 μl PBS/Matrigel (50: 50) into the right forelimb to establish xenograft tumors. From the first day, mice were intraperitoneally injected with 10 mg/kg Doc every three days. Tumor volumes were monitored by direct measurement with calipers and calculated by the formula: (length × width²)/2. **P < 0.01 vs. Doc + LV-miR-Ctl; ^# P < 0.05, ^## P < 0.01 vs. LV-anti-miR-193a-5p (each groups, n = 16). b Representative tumor sizes in each group of mice. c Xenograft tumor wet weight in each group of mice. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Doc + LV-anti-miR-193a-5p. d The xenograft models of nude mice were prepared as in (A), Western blotting detected the expression of Bach2, HO-1, cleaved caspase-3, Bcl-2 and Bax in xenograft tumors. e The xenograft models of nude mice were prepared as in (a), the TUNEL staining detected cell apoptosis in xenograft tumors. Blue staining represents the nucleus, and red staining indicates TUNEL-positive cells