Fig. 5

miR-498 targets HMGA2 and negatively regulates HMGA2 expression. A Venn diagram of the downstream target genes of miR-498 predicted by StarBase and miRDB predict and BCa-related genes obtained from the GeneCards database; B A box diagram of HMGA2 expression in BCa compared with normal samples analyzed by StarBase website; C Putative miR-498 binding sites in the 3′-UTR of HMGA2 mRNA predicted by the StarBase website; D The targeting relationship between miR-498 and HMGA2 determined with dual-luciferase reporter gene assay. E The expression of HMGA2 in BCa cells determined with RT-qPCR; F Representative Western blots of HMGA2 protein and its quantitation in the BCa cells. *p < 0.05 vs. mimic NC. # p < 0.05 vs. inhibitor NC. All cell experiments were repeated three times