Fig. 5

FMRP interacts with circZNF609 in acral melanoma and cutaneous melanoma. a Venn diagram showed potential downstream RBP targets of circZNF609 according to bioinformatic analysis results. b and c The co-localization of circZNF609 and FMRP protein in HMY-1 cells (b) and A875 cells (c) by immunofluorescence and FISH. d Identification of the circZNF609-FMRP complex pulled down by circZNF609 junction probe with protein extracts from HMY-1 cells. e Immunoblot analysis of FMRP after pulldown assay with circZNF609 specific junction probe in HMY-1 cells. f RIP assays showed the association of FMRP with circZNF609 in HMY-1 cells. g Identification of the circZNF609-FMRP complex pulled down by circZNF609 junction probe with protein extracts from A875 cells. h Immunoblot analysis of FMRP after pulldown assay with circZNF609 specific junction probe in A875 cells. i RIP assays showed the association of FMRP with circZNF609 in A875 cells