Fig. 1

Wild-type IDH1 is overexpressed in primary and metastatic melanoma. A RNA- sequencing data showing expression of IDH1 in human primary melanoma compared to that in normal skin tissues, *P < 0.05. B IDH1 RNA expression in human primary melanoma compared to metastatic melanoma *P < 0.05. C Correlation between IDH1 expression and overall survival rate of melanoma patients by Kaplan-Meier analysis using the log-rank test P < 0.035. The data of A, B, and C were obtained from the TCGA database. D IDH1 mRNA expression level in different melanoma cell lines by qPCR. Expression levels are normalized to 18S expression in each cell line. E Representative immunoblot analysis of IDH1 in different melanoma cell lines and primary human melanocytes; alpha-tubulin used for normalization of cellular protein. The relative protein level of IDH1 across three experiments is quantified by densitometry. Each data point represents the mean ± SEM of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001)