Fig. 7
Knockdown of cZNF215 boosts anti-tumor effect of ipatasertib in vivo. A RBE cells (stably expressing sh-NC vector or sh-cZNF215) were used to establish subcutaneous xenograft models. The figure showed representative images of excised tumors at the end of the experiment. B and C Tumor volume and tumor weight of subcutaneous xenografts. D and E Representative bioluminescent images of liver orthotopic-implantation models, and quantitative analysis of bioluminescent results and Ki-67 staining results. F Representative fluorescent images of lung metastatic nodules (top); representative HE-stained images of lung metastatic nodules and IHC staining of p-AKTSer473/Thr308 and Ki-67 in lung metastases (bottom). G Quantitative analysis of bioluminescent results, and staining results of p-AKTSer473/Thr308 and Ki-67. H Summary figure of the mechanism that how cZNF215 facilitates iCCA growth and metastasis