Fig. 1

EFTUD2 is remarkably upregulated in CRC specimens. A Analysis of EFTUD2 mRNA expression in CRC tissues compared with adjacent normal tissues using TCGA database (Normal tissue, n = 101; Tumor tissue, n = 697). B Analysis of EFTUD2 protein expression in CRC tissues compared with paired adjacent normal tissues using CPTAC (Normal tissue, n = 96; Tumor tissue, n = 96). C RT-qPCR analysis of EFTUD2 mRNA expression in CRC tissues compared with adjacent normal tissues (Normal tissue, n = 66; Tumor tissue, n = 66). GAPDH is used as an endogenous control. D Representative images of EFTUD2 expression in 34 paired adjacent normal versus CRC tissue samples from the Nanchang Hospital cohort using IHC staining. E Quantification of EFTUD2 levels in CRC tissues and their corresponding adjacent normal tissues using IHC staining. F Representative images of EFTUD2 expression in CRC versus adjacent normal tissues using IF staining. G Quantification of EFTUD2 levels in CRC tissues and their corresponding adjacent normal tissues using IF staining. H Western blotting analysis of EFTUD2 protein expression in CRC tissues and their corresponding adjacent normal tissues. β-Actin is used as a loading control. I Quantification of the protein levels of EFTUD2 in CRC tissues and their corresponding adjacent normal tissues using Western blotting. Each bar represents the mean values ± SD. **P < 0.01; ***P < 0.001