Fig. 3

EFTUD2 attenuates the chemotherapy efficacy of 5-FU in vitro.  A Western blotting and RT-qPCR analyses of EFTUD2 expression in five CRC cell lines and normal colonic epithelial cells. β-Actin is used as a loading control in the Western blotting, and GAPDH serves as an endogenous control in RT-qPCR. B-D Western blotting and RT-qPCR analyses of changes in EFTUD2 expression levels in SW480, HCT116, and Caco-2 following overexpression or knockdown of EFTUD2. E–G MTT assay showing the effect of EFTUD2 overexpression or knockdown on cell viability in CRC cell lines exposed to a gradient of 5-FU concentrations. H and I Clone formation (H) and EdU (I) assays showing the effect of EFTUD2 on survival and proliferation potential in CRC cell lines exposed to 5-FU. Each bar represents the mean values ± SD. *P < 0.05; **P < 0.01; ***P < 0.001