Fig. 4

EFTUD2 hampers the chemotherapy efficacy of 5-FU in vivo. A and B HCT116 cells transfected with sh-EFTUD2 are subcutaneously injected into BALB/c nude mice, and tumor volumes are assessed using an animal live imaging system (IVIS) (A). Representative images of tumors harvested from different groups of mice (n = 5) are displayed (B). C and D Similarly, SW480 cells transfected with p-EFTUD2 are subcutaneously injected (C), and representative images of tumors from these mice (n = 5) are presented (D). E and G Growth curves illustrating the changes in tumor volume in mice across various groups (volume = 0.52 × length × width²) (n = 5). F and H Differences in tumor weight between the groups (n = 5). Each bar represents the mean values ± SD, **P < 0.01, ***P < 0.001