Fig. 5

EFTUD2 interacts with c-MYC and increases c-MYC protein abundance in CRC. A Protein-protein interaction analysis between EFTUD2 and c-MYC protein using EMBL-EBI database. B and C Co-IP and Western blotting analyses detecting the interaction between EFTUD2 and c-MYC by modulating their exogenous (B) and endogenous (C) expression in SW480 and HCT116 cells. D IF staining showing co-localization of EFTUD2 and c-MYC in SW480 and HCT116 cells. DAPI (blue), EFTUD2 (green), and c-MYC (red). E and F Western blotting analysis of the impact of overexpression or knockdown of EFTUD2 on the expression of c-MYC in SW480 and HCT116 cells